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phosphoramidite-oligonucleotide-dye-modification
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The phosphoramidite method is one of the strategies of nucleic acid chemical synthesis. Each cycle of the synthesis of nucleic acid chains is through high-efficiency and chemically active nucleotide 3'-phosphoramidite intermediates, which become stable pentavalent phosphate triesters after oxidation. The phosphoramidite method is currently the most commonly used DNA automated solid-phase synthesis method. phosphoramidite-oligonucleotide-dye-modification https://probes.bocsci.com/reactive-group/phosphoramidite.html